SampleNames: Prx6_AC1-1 Prx6_AC2-1 Prx6_AC3-1 Prx6_AC4-1 Prx6_AC5-1 Prx6_AC6-1 Prx6_AC7-1 Prx6_AC8-1 Prx6_AC1-2 Prx6_AC2-2 Prx6_AC3-2 Prx6_AC4-2 Prx6_AC5-2 Prx6_AC6-2 Prx6_AC7-2 Prx6_AC8-2 Prx6_AV1-1 Prx6_AV2-1 Prx6_AV3-1 Prx6_AV4-1 Prx6_AV5-1 Prx6_AV6-1 Prx6_AV7-1 Prx6_AV8-1 Prx6_AV1-2 Prx6_AV2-2 Prx6_AV3-2 Prx6_AV4-2 Prx6_AV5-2 Prx6_AV6-2 Prx6_AV7-2 Prx6_blank1 Prx6_CC1-1 Prx6_CC2-1 Prx6_CC3-1 Prx6_CC4-1 Prx6_CC5-1 Prx6_CC6-1 Prx6_CC7-1 Prx6_CC8-1 Prx6_CC1-2 Prx6_CC2-2 Prx6_CC3-2 Prx6_CC4-2 Prx6_CC5-2 Prx6_CC6-2 Prx6_CC7-2 Prx6_blank2 Prx6_CV1-1 Prx6_CV2-1 Prx6_CV3-1 Prx6_CV4-1 Prx6_CV5-1 Prx6_CV6-1 Prx6_CV7-1 Prx6_CV8-1 Prx6_CV1-2 Prx6_CV2-2 Prx6_CV3-2 Prx6_CV4-2 Prx6_CV5-2 Prx6_CV6-2 Prx6_CV7-2 Prx6_blank3 Logistic_a: 0.264364 0.305768 0.338722 0.442239 0.380498 0.393372 0.511039 0.679186 0.335306 0.612679 0.450845 0.532699 0.452928 0.580695 0.591615 0.511639 0.448335 0.688578 0.676129 0.600852 0.445428 0.466325 0.591243 0.567849 0.527057 0.497788 0.786356 0.609735 0.527462 0.410737 0.589955 0.00628691 0.432824 0.778002 0.636882 0.619687 0.629862 0.506109 0.80327 0.769815 0.442224 0.566174 0.656316 0.638558 0.589817 0.464863 0.42196 0.00472268 0.505419 0.529992 0.597606 0.569362 0.589754 0.538387 0.687987 0.595506 0.354489 0.400311 0.445076 0.417621 0.521773 0.539591 0.353516 0.004385 Logistic_b: -12.9494 -13.5335 -12.1662 -13.4834 -13.2655 -13.3134 -13.7003 -12.7311 -13.2074 -13.7902 -12.3287 -13.5635 -13.4152 -13.041 -13.594 -12.5716 -13.2383 -12.8056 -12.9922 -13.4758 -13.2873 -13.2342 -13.0333 -12.6242 -13.5431 -13.064 -12.9488 -13.2126 -13.1218 -13.3293 -13.0168 -21.2213 -13.2683 -12.6502 -12.5619 -13.0961 -12.5707 -12.3119 -13.0338 -12.9839 -13.2369 -12.6679 -12.5136 -13.3154 -12.6092 -12.3246 -12.7953 -23.4042 -13.0215 -13.045 -12.6457 -13.2789 -12.1972 -13.2747 -12.0615 -13.9899 -12.9037 -13.191 -12.7455 -12.913 -11.9069 -13.1552 -12.0968 -42.491 Logistic_X0: 29.7429 30.6245 28.6935 32.1752 30.7645 32.0082 33.574 30.6585 29.8065 30.9516 28.9183 32.0593 30.9814 31.9068 33.3248 30.5421 30.6352 29.5035 29.5276 30.5914 30.9923 31.9203 31.053 30.1054 30.6856 29.5883 29.7508 30.6502 30.9585 32.0411 31.0172 31.9081 30.7954 29.3826 29.0058 31.6478 30.1215 29.747 31.4939 30.6664 30.8366 29.3475 29.3464 31.8151 30.1614 29.8811 31.3435 34.8063 30.4326 30.6134 29.758 30.8513 29.5004 32.7097 29.4568 31.7817 30.5981 30.789 29.7733 30.9803 29.3767 32.7384 29.4323 31.6323 Logistic_Y0: 0.000997451 0.000897475 0.000593123 -9.23398e-005 0.000857898 0.00252598 0.000626017 0.00160239 0.00162866 0.0036659 0.000932079 0.00075614 0.00163277 0.00111405 0.000679678 0.00139258 0.00139631 0.0018179 0.0023464 0.00115867 0.000734809 0.00225695 0.00152931 0.000622524 0.000224282 0.000906727 0.00127426 0.000682977 0.00109962 0.00111172 0.000874097 0.00208834 0.0013215 0.00175021 0.00121805 0.00107359 0.00157824 0.000408465 0.000677567 0.00149524 0.000721588 0.00201833 0.00146796 0.00449231 0.00383682 0.00333498 0.00089595 0.00157942 0.000553152 0.000617554 0.000516131 0.000371003 0.000895137 0.000622156 0.00155131 0.00060186 0.00118205 0.00135631 0.0012566 0.000586518 0.000594582 1.50689e-005 0.000541387 0.00266423 Logistic_Pvalue: 7.72236e-082 4.90833e-088 1.21349e-081 4.36137e-085 5.06513e-088 8.31521e-077 1.20476e-084 6.99476e-085 7.19918e-082 1.60843e-076 6.85207e-083 5.96941e-090 4.27321e-083 1.35845e-082 2.80089e-089 8.56955e-079 7.94191e-093 1.05537e-084 2.62884e-086 3.2119e-081 2.0367e-084 5.31092e-072 9.26452e-079 1.18641e-084 7.42711e-083 1.06233e-082 3.59907e-080 1.10286e-084 6.75653e-084 7.54132e-085 7.64524e-085 2.16052e-013 2.10504e-085 1.88372e-082 1.418e-087 7.9636e-086 3.96041e-079 2.13282e-092 1.57803e-089 1.46856e-087 2.49864e-090 4.9483e-080 2.68534e-082 9.78223e-076 8.75142e-079 9.5143e-075 2.95183e-086 1.21423e-016 2.80534e-088 1.67379e-093 1.29336e-085 8.59321e-082 7.81666e-084 5.44361e-089 3.59554e-085 1.386e-079 2.69642e-081 1.52234e-086 8.63521e-087 4.93837e-085 8.6182e-089 1.03919e-082 1.23397e-079 9.97111e-013 Noise(SPE): 0.000249568 0.000196497 0.000336944 0.000312302 0.000243171 0.000445761 0.00034407 0.000519924 0.000315206 0.000741319 0.000414502 0.000287928 0.000378148 0.000477535 0.000311194 0.000554069 0.00021999 0.000558787 0.000500956 0.000567001 0.000345061 0.000695029 0.000627474 0.000450853 0.000451883 0.000450542 0.000815014 0.000472344 0.000421299 0.000295803 0.000445599 0.000289131 0.000320006 0.000719917 0.000448549 0.000430327 0.000681515 0.000263953 0.000456308 0.000506955 0.000247484 0.000600999 0.00061347 0.000775617 0.000649571 0.00064972 0.000289972 0.000150404 0.00032292 0.000250562 0.000456026 0.00051434 0.000502565 0.000297116 0.000544803 0.000584247 0.00033284 0.000277657 0.000317746 0.000312569 0.000338243 0.000422991 0.000382408 0.000214874 EndofExpPhase(SDM): 0.0468844 0.0544585 0.0585837 0.0773161 0.0672375 0.0712025 0.0903641 0.119063 0.0600712 0.111392 0.0783512 0.0941101 0.0808304 0.102059 0.104405 0.0896337 0.0795772 0.121053 0.119788 0.106318 0.078468 0.0835697 0.104295 0.0986476 0.0925613 0.0874714 0.137765 0.106966 0.0929096 0.0728376 0.103388 0.00327081 0.0768329 0.136114 0.111041 0.108892 0.110207 0.087291 0.140297 0.135191 0.0778352 0.0998281 0.114546 0.115978 0.105628 0.0831554 0.0739502 0.00247779 0.0883847 0.0927543 0.103717 0.0997194 0.101922 0.0945597 0.119104 0.105603 0.0626661 0.071111 0.0782482 0.0730317 0.0894798 0.0939861 0.0609855 0.00353965 CP(SPE): 17.3705 17.7921 16.2572 18.7864 17.671 19.2327 19.702 17.4509 17.5859 19.0186 16.4025 18.4116 18.2667 18.5077 19.1238 17.7442 17.2292 16.9256 16.9559 18.2448 18.0782 19.5221 18.3629 17.1039 18.2185 17.306 17.5005 17.8243 17.978 18.6195 17.8564 27.6596 17.8864 16.9164 16.2769 18.1634 17.4979 16.1002 17.7514 17.4444 17.5146 17.0936 16.8076 19.2182 17.5749 17.5318 17.7409 30.0814 17.2995 17.0221 16.8698 18.1994 16.5274 18.5889 16.2962 19.372 17.8284 17.7397 16.8652 17.7436 15.8585 19.0108 16.7373 29.4998 CP(SDM): 26.3662 27.3116 25.2049 28.6804 27.3629 28.483 29.9903 27.1124 26.4949 27.6714 25.4533 28.5996 27.5976 28.3123 29.7373 26.9602 27.2402 26.1127 26.1874 27.2666 27.5716 28.3817 27.5524 26.5909 27.3687 26.2614 26.373 27.2464 27.4944 28.5169 27.5158 29.7832 27.3911 25.9602 25.6012 28.0989 26.5887 26.1774 27.9438 27.195 27.4189 25.9344 25.8872 28.3118 26.6357 26.2994 27.738 32.7173 26.9986 27.1658 26.2905 27.4438 25.9238 29.0957 25.8412 28.4659 27.1108 27.3637 26.3338 27.4522 25.7193 29.0853 25.8313 30.6153 DynamicThreshold: 0.0240657 0.0277762 0.0297569 0.038768 0.0341693 0.0370871 0.0456671 0.0605926 0.0310075 0.0578994 0.0398489 0.0475771 0.0414206 0.0518252 0.0526981 0.0457902 0.0405967 0.0617146 0.0613175 0.0540219 0.0397739 0.0432608 0.0532258 0.0498605 0.0466187 0.0444143 0.069927 0.0540607 0.0472153 0.0371226 0.052354 0.00282414 0.0392372 0.0692919 0.0563536 0.0551979 0.0562335 0.0439817 0.0707156 0.0685967 0.0394021 0.0512237 0.0583136 0.0606229 0.0550571 0.0435701 0.0375681 0.0021038 0.0446304 0.0468112 0.0523444 0.0503024 0.0516599 0.0477395 0.0605999 0.0533948 0.0320905 0.0363725 0.0399113 0.0369654 0.0452063 0.0472121 0.0309547 0.00320938 LowerCycleNumber: 17 18 16 19 18 19 20 17 18 19 16 18 18 19 19 18 17 17 17 18 18 20 18 17 18 17 18 18 18 19 18 Error! 18 17 16 18 17 16 18 17 18 17 17 19 18 18 18 Error! 17 17 17 18 17 19 16 19 18 18 17 18 16 19 17 Error! UpperCycleNumber: 26 27 25 29 27 28 30 27 26 28 25 29 28 28 30 27 27 26 26 27 28 28 28 27 27 26 26 27 27 29 28 Error! 27 26 26 28 27 26 28 27 27 26 26 28 27 26 28 Error! 27 27 26 27 26 29 26 28 27 27 26 27 26 29 26 Error! PointsForRegression: 10 10 10 11 10 10 11 11 9 10 10 12 11 10 12 10 11 10 10 10 11 9 11 11 10 10 9 10 10 11 11 Error! 10 10 11 11 11 11 11 11 10 10 10 10 10 9 11 Error! 11 11 10 10 10 11 11 10 10 10 10 10 11 11 10 Error! Number_OfRegressionWindows: 779 785 721 1593 791 765 1641 1619 328 752 765 3661 1703 789 3680 715 1721 671 788 800 1707 310 1660 1704 587 746 325 779 765 1681 1721 Error! 716 764 1661 1717 1589 1651 1730 1706 746 776 781 760 758 318 1673 Error! 1646 1679 781 685 776 1650 1728 781 791 765 790 758 1719 1702 761 Error! WeightedAverage_OfPvalue: 0.00230269 0.00237224 0.00287777 0.00241069 0.00229134 0.00256603 0.00230036 0.00230427 0.00351038 0.00275252 0.00267929 0.00130665 0.00189911 0.00230917 0.00112674 0.00308579 0.00171162 0.0033313 0.0021908 0.00228586 0.00186271 0.00403056 0.00203867 0.00183406 0.00324154 0.00274799 0.003407 0.00231233 0.00254999 0.00217835 0.00167136 Error! 0.00308275 0.00264391 0.00202675 0.00176264 0.00222972 0.00202759 0.00168729 0.00190696 0.00294589 0.0023251 0.00246577 0.00269979 0.00272072 0.00348484 0.0015901 Error! 0.00219482 0.00187818 0.00226776 0.0028999 0.00242107 0.00218471 0.00157975 0.00241906 0.00227286 0.00250749 0.00226993 0.00268403 0.00172686 0.00185432 0.0025641 Error! Stderr_OfWeightedPvalue: 0.000352785 0.000379788 0.000348887 0.000212873 0.000349209 0.000350886 0.000188032 0.000208939 0.000698556 0.000398254 0.000344674 0.000120079 0.000205907 0.000366797 0.000110521 0.000382011 0.000200985 0.000383831 0.000348111 0.000386472 0.000226348 0.000692573 0.000203385 0.000218818 0.000389948 0.000371538 0.000690297 0.000335729 0.000369032 0.000227125 0.000218002 Error! 0.000358651 0.000387207 0.000225943 0.000209496 0.000206547 0.000211687 0.000210798 0.000213447 0.000383447 0.000389378 0.000372599 0.000395233 0.000389365 0.000609976 0.000209768 Error! 0.000196493 0.000211088 0.00033892 0.00041022 0.00038738 0.000219223 0.00018474 0.000345984 0.000360189 0.00034851 0.000370235 0.00034879 0.000210224 0.000220652 0.000409853 Error! WeightedAverage_OfEfficiency: 0.727219 0.718801 0.851251 0.780951 0.73205 0.691841 0.807356 0.789799 0.817245 0.749001 0.745145 0.724968 0.700715 0.682385 0.698023 0.825609 0.667098 0.845711 0.702529 0.707577 0.65707 0.80456 0.728163 0.692126 0.869266 0.818314 0.816056 0.735466 0.804084 0.707826 0.702484 Error! 0.820485 0.790511 0.727173 0.703851 0.818976 0.853395 0.703675 0.715181 0.836863 0.694951 0.666813 0.751201 0.725884 0.791175 0.626968 Error! 0.834984 0.761815 0.774289 0.833877 0.701159 0.762351 0.661032 0.825392 0.66341 0.813159 0.687935 0.769439 0.763924 0.742372 0.664947 Error! Stderr_OfWeightedEfficiency: 0.00351705 0.00373304 0.00547395 0.00361674 0.00312202 0.00469627 0.0034395 0.0035969 0.00663626 0.00450438 0.00433653 0.00163167 0.00244424 0.00270717 0.00126503 0.00518421 0.00202544 0.00625992 0.00289359 0.00272087 0.0022594 0.00704004 0.0030876 0.00247389 0.00651826 0.00456408 0.007454 0.00384612 0.0044126 0.00327529 0.00231848 Error! 0.00507767 0.00471187 0.00320614 0.00221817 0.00403431 0.00360246 0.00196739 0.00252109 0.00501695 0.00318663 0.00353327 0.00432639 0.00435516 0.00659515 0.00231867 Error! 0.00365172 0.00309743 0.00439091 0.00544556 0.00342857 0.00306721 0.00182268 0.00410801 0.0030663 0.00405431 0.00288631 0.00405963 0.00272799 0.00249118 0.00362235 Error! CT: 24.8114 25.7629 23.6298 27.0492 25.7817 26.8489 28.3095 25.4856 24.9612 26.1364 23.8813 26.9799 26.0219 26.6522 28.0566 25.3274 25.6613 24.555 24.6455 25.7178 25.9825 26.7488 25.9389 24.9831 25.8203 24.7251 24.8188 25.6677 25.8912 26.8775 25.8987 29.0099 25.8099 24.3952 24.045 26.4562 24.9782 24.5536 26.3012 25.5918 25.8303 24.3746 24.3107 26.6887 25.0273 24.6808 26.0806 31.8461 25.4111 25.569 24.7031 25.8627 24.3058 27.4144 24.2106 26.9054 25.5067 25.7747 24.7548 25.8266 24.0749 27.3933 24.2095 30.2119 TotalSampleNumber: 64 ReplicateSampleNames: Prx6_AC1-1 Prx6_AC2-1 Prx6_AC3-1 Prx6_AC4-1 Prx6_AC5-1 Prx6_AC6-1 Prx6_AC7-1 Prx6_AC8-1 Prx6_AC1-2 Prx6_AC2-2 Prx6_AC3-2 Prx6_AC4-2 Prx6_AC5-2 Prx6_AC6-2 Prx6_AC7-2 Prx6_AC8-2 Prx6_AV1-1 Prx6_AV2-1 Prx6_AV3-1 Prx6_AV4-1 Prx6_AV5-1 Prx6_AV6-1 Prx6_AV7-1 Prx6_AV8-1 Prx6_AV1-2 Prx6_AV2-2 Prx6_AV3-2 Prx6_AV4-2 Prx6_AV5-2 Prx6_AV6-2 Prx6_AV7-2 Prx6_blank1 Prx6_CC1-1 Prx6_CC2-1 Prx6_CC3-1 Prx6_CC4-1 Prx6_CC5-1 Prx6_CC6-1 Prx6_CC7-1 Prx6_CC8-1 Prx6_CC1-2 Prx6_CC2-2 Prx6_CC3-2 Prx6_CC4-2 Prx6_CC5-2 Prx6_CC6-2 Prx6_CC7-2 Prx6_blank2 Prx6_CV1-1 Prx6_CV2-1 Prx6_CV3-1 Prx6_CV4-1 Prx6_CV5-1 Prx6_CV6-1 Prx6_CV7-1 Prx6_CV8-1 Prx6_CV1-2 Prx6_CV2-2 Prx6_CV3-2 Prx6_CV4-2 Prx6_CV5-2 Prx6_CV6-2 Prx6_CV7-2 Prx6_blank3 AverageEfficiency_OfReplicateSamples 0.727219 0.718801 0.851251 0.780951 0.73205 0.691841 0.807356 0.789799 0.817245 0.749001 0.745145 0.724968 0.700715 0.682385 0.698023 0.825609 0.667098 0.845711 0.702529 0.707577 0.65707 0.80456 0.728163 0.692126 0.869266 0.818314 0.816056 0.735466 0.804084 0.707826 0.702484 Error! 0.820485 0.790511 0.727173 0.703851 0.818976 0.853395 0.703675 0.715181 0.836863 0.694951 0.666813 0.751201 0.725884 0.791175 0.626968 Error! 0.834984 0.761815 0.774289 0.833877 0.701159 0.762351 0.661032 0.825392 0.66341 0.813159 0.687935 0.769439 0.763924 0.742372 0.664947 Error! StdErr_OfReplicateSamplesEfficiency: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! CoefficientVariation(CV%)_OfReplicateSamplesEfficiencies: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Error! AverageCT_OfReplicateSamples: 24.8114 25.7629 23.6298 27.0492 25.7817 26.8489 28.3095 25.4856 24.9612 26.1364 23.8813 26.9799 26.0219 26.6522 28.0566 25.3274 25.6613 24.555 24.6455 25.7178 25.9825 26.7488 25.9389 24.9831 25.8203 24.7251 24.8188 25.6677 25.8912 26.8775 25.8987 29.0099 25.8099 24.3952 24.045 26.4562 24.9782 24.5536 26.3012 25.5918 25.8303 24.3746 24.3107 26.6887 25.0273 24.6808 26.0806 31.8461 25.4111 25.569 24.7031 25.8627 24.3058 27.4144 24.2106 26.9054 25.5067 25.7747 24.7548 25.8266 24.0749 27.3933 24.2095 30.2119 StdErr_OfReplicateSamplesCT: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CV%_OfReplicateSamplesCTs: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 GeneNames Prx6 AverageEfficiency_OfGenes: 0.750621 Stdev_OfGenesEfficiency: 0.00784249 MeanCV%_OfGenesEfficiencies -49.4754 MeanCV%_OfGenesCTs 0 Some problems are found! ------Please check your data and re-run Realtime PCR Miner (Version 2.0, Author: Dr. Sheng Zhao, Email: windupzs@gmail.com). Error#1: Sample#32, Exponential phase fit failed! Error#2: Sample#48, Exponential phase fit failed! Error#3: Sample#64, Exponential phase fit failed!